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Promega pf3a wg bydv
Pf3a Wg Bydv, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pf3a+wg+bydv/pf3a/pmc10242721__pnas__2221308120__sapp-15-32-35
Average 90 stars, based on 1 article reviews
pf3a wg bydv - by Bioz Stars, 2026-09
90/100 stars

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Expressing:

Article Title: Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT
Article Snippet: The S132A substitution was introduced into this vector using QuikChange mutagenesis (Agilent) according to the manufacturer’s instructions. .. CERT L destined for expression using in vitro transcription/translation system from wheat germ extract was cloned into the plasmid pF3A WG BYDV (Promega) with an N-terminal myc epitope tag. ..

Article Title: Low-oxygen response is triggered by an ATP-dependent shift in oleoyl-CoA in Arabidopsis
Article Snippet: .. For in vitro expression, the CDS of ACBP1 was fused N terminally with a Flag tag by PCR and cloned into pF3A WG BYDV (Promega), while the CDS of RAP2.12 was fused C terminally to CFP by PCR and cloned into pF3A WG BYDV (Promega). ..

Article Title:
Article Snippet: .. For in vitro expression, the CDS of ANAC013, ANAC016 and ANAC017 was fused N-terminally with a Flag tag by PCR with oligonucleotides containing restriction site overhangs (Supplemental Table 5), and cloned into pF3A WG BYDV (Promega). .. The ANAC13 reporter constructs were synthesized by GeneArt (Thermo Fisher Scientific) based on CDS sequences (Supplemental Fig. 5).

In Vitro:

Article Title: Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT
Article Snippet: The S132A substitution was introduced into this vector using QuikChange mutagenesis (Agilent) according to the manufacturer’s instructions. .. CERT L destined for expression using in vitro transcription/translation system from wheat germ extract was cloned into the plasmid pF3A WG BYDV (Promega) with an N-terminal myc epitope tag. ..

Article Title: ERFVII-controlled hypoxia responses are in part facilitated by MEDIATOR SUBUNIT 25 in Arabidopsis thaliana.
Article Snippet: For BiFC analysis, MED25 coding sequences from Arabidopsis and rice were recombined into pDH51-GW-YFPn, whilst fulllength RAP2.2, RAP2.12 and SUB1A coding sequences in pENTR/D-TOPO (Invitrogen) were recombined into pDH51-GWYFPc (Zhong et al., 2008). .. For in vitro pulldown experiments the coding region of AtMED25Dq was fused N-terminally with a FLAG-tag by PCR with oligonucleotides containing restriction site overhangs for cloning into pF3A WG BYDV (Promega, Fitchburg, WI, USA). ..

Article Title: Low-oxygen response is triggered by an ATP-dependent shift in oleoyl-CoA in Arabidopsis
Article Snippet: .. For in vitro expression, the CDS of ACBP1 was fused N terminally with a Flag tag by PCR and cloned into pF3A WG BYDV (Promega), while the CDS of RAP2.12 was fused C terminally to CFP by PCR and cloned into pF3A WG BYDV (Promega). ..

Article Title:
Article Snippet: .. For in vitro expression, the CDS of ANAC013, ANAC016 and ANAC017 was fused N-terminally with a Flag tag by PCR with oligonucleotides containing restriction site overhangs (Supplemental Table 5), and cloned into pF3A WG BYDV (Promega). .. The ANAC13 reporter constructs were synthesized by GeneArt (Thermo Fisher Scientific) based on CDS sequences (Supplemental Fig. 5).

Clone Assay:

Article Title: Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT
Article Snippet: The S132A substitution was introduced into this vector using QuikChange mutagenesis (Agilent) according to the manufacturer’s instructions. .. CERT L destined for expression using in vitro transcription/translation system from wheat germ extract was cloned into the plasmid pF3A WG BYDV (Promega) with an N-terminal myc epitope tag. ..

Article Title: Low-oxygen response is triggered by an ATP-dependent shift in oleoyl-CoA in Arabidopsis
Article Snippet: .. For in vitro expression, the CDS of ACBP1 was fused N terminally with a Flag tag by PCR and cloned into pF3A WG BYDV (Promega), while the CDS of RAP2.12 was fused C terminally to CFP by PCR and cloned into pF3A WG BYDV (Promega). ..

Article Title:
Article Snippet: .. For in vitro expression, the CDS of ANAC013, ANAC016 and ANAC017 was fused N-terminally with a Flag tag by PCR with oligonucleotides containing restriction site overhangs (Supplemental Table 5), and cloned into pF3A WG BYDV (Promega). .. The ANAC13 reporter constructs were synthesized by GeneArt (Thermo Fisher Scientific) based on CDS sequences (Supplemental Fig. 5).

Plasmid Preparation:

Article Title: Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT
Article Snippet: The S132A substitution was introduced into this vector using QuikChange mutagenesis (Agilent) according to the manufacturer’s instructions. .. CERT L destined for expression using in vitro transcription/translation system from wheat germ extract was cloned into the plasmid pF3A WG BYDV (Promega) with an N-terminal myc epitope tag. ..

FLAG-tag:

Article Title: ERFVII-controlled hypoxia responses are in part facilitated by MEDIATOR SUBUNIT 25 in Arabidopsis thaliana.
Article Snippet: For BiFC analysis, MED25 coding sequences from Arabidopsis and rice were recombined into pDH51-GW-YFPn, whilst fulllength RAP2.2, RAP2.12 and SUB1A coding sequences in pENTR/D-TOPO (Invitrogen) were recombined into pDH51-GWYFPc (Zhong et al., 2008). .. For in vitro pulldown experiments the coding region of AtMED25Dq was fused N-terminally with a FLAG-tag by PCR with oligonucleotides containing restriction site overhangs for cloning into pF3A WG BYDV (Promega, Fitchburg, WI, USA). ..

Article Title: Low-oxygen response is triggered by an ATP-dependent shift in oleoyl-CoA in Arabidopsis
Article Snippet: .. For in vitro expression, the CDS of ACBP1 was fused N terminally with a Flag tag by PCR and cloned into pF3A WG BYDV (Promega), while the CDS of RAP2.12 was fused C terminally to CFP by PCR and cloned into pF3A WG BYDV (Promega). ..

Article Title:
Article Snippet: .. For in vitro expression, the CDS of ANAC013, ANAC016 and ANAC017 was fused N-terminally with a Flag tag by PCR with oligonucleotides containing restriction site overhangs (Supplemental Table 5), and cloned into pF3A WG BYDV (Promega). .. The ANAC13 reporter constructs were synthesized by GeneArt (Thermo Fisher Scientific) based on CDS sequences (Supplemental Fig. 5).

Polymerase Chain Reaction:

Article Title: ERFVII-controlled hypoxia responses are in part facilitated by MEDIATOR SUBUNIT 25 in Arabidopsis thaliana.
Article Snippet: For BiFC analysis, MED25 coding sequences from Arabidopsis and rice were recombined into pDH51-GW-YFPn, whilst fulllength RAP2.2, RAP2.12 and SUB1A coding sequences in pENTR/D-TOPO (Invitrogen) were recombined into pDH51-GWYFPc (Zhong et al., 2008). .. For in vitro pulldown experiments the coding region of AtMED25Dq was fused N-terminally with a FLAG-tag by PCR with oligonucleotides containing restriction site overhangs for cloning into pF3A WG BYDV (Promega, Fitchburg, WI, USA). ..

Article Title: Low-oxygen response is triggered by an ATP-dependent shift in oleoyl-CoA in Arabidopsis
Article Snippet: .. For in vitro expression, the CDS of ACBP1 was fused N terminally with a Flag tag by PCR and cloned into pF3A WG BYDV (Promega), while the CDS of RAP2.12 was fused C terminally to CFP by PCR and cloned into pF3A WG BYDV (Promega). ..

Article Title:
Article Snippet: .. For in vitro expression, the CDS of ANAC013, ANAC016 and ANAC017 was fused N-terminally with a Flag tag by PCR with oligonucleotides containing restriction site overhangs (Supplemental Table 5), and cloned into pF3A WG BYDV (Promega). .. The ANAC13 reporter constructs were synthesized by GeneArt (Thermo Fisher Scientific) based on CDS sequences (Supplemental Fig. 5).

Cloning:

Article Title: ERFVII-controlled hypoxia responses are in part facilitated by MEDIATOR SUBUNIT 25 in Arabidopsis thaliana.
Article Snippet: For BiFC analysis, MED25 coding sequences from Arabidopsis and rice were recombined into pDH51-GW-YFPn, whilst fulllength RAP2.2, RAP2.12 and SUB1A coding sequences in pENTR/D-TOPO (Invitrogen) were recombined into pDH51-GWYFPc (Zhong et al., 2008). .. For in vitro pulldown experiments the coding region of AtMED25Dq was fused N-terminally with a FLAG-tag by PCR with oligonucleotides containing restriction site overhangs for cloning into pF3A WG BYDV (Promega, Fitchburg, WI, USA). ..



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